Abstract
The molecular mechanisms by which the tight junction integral membrane protein, occludin promotes cell adhesion and establishes an endothelial monolayer permeability barrier have not been elucidated. In particular, the amino acid sequences of the occludin cell adhesion recognition (CAR) sites have not been determined. Here we demonstrate that a cyclic peptide containing the sequence LYHY, which is found in the second extracellular domain of occludins in all mammalian species, inhibits the establishment of endothelial cell barriers in vitro and in vivo. This cyclic peptide also prevents the aggregation of fibroblasts stably transfected with cDNA encoding occludin. The data suggest that the LYHY motif is an occludin CAR sequence.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Tsukita, S., M. Furuse, and M. Itoh. 1999. Curr. Opin. Cell Biol. 11:628–633.
Vestweber, D. 2000. J. Pathol. 190:281–291.
Furuse, M., T. Hirase, M. Itoh, A. Nagafuchi, S. Yonemura, S. Tsukita, and S. Tsukita. 1993. J. Cell Biol. 123:1777–1788.
Furuse, M., M. Itoh, T. Hirase, A. Nagafuchi, S. Yonemura, S. Tsukita, and S. Tsukita. 1994. J. Cell Biol. 127:1617–1626.
Van Itallie, C. M. and J. M. Anderson. 1997. J. Cell Sci. 110(Pt 9):1113–1121.
Lacaz-Vieira, F., M. M. Jaeger, P. Farshori, and B. Kachar. 1999. J. Membr. Biol. 168:289–297.
Wong, V. and B. M. Gumbiner. 1997. J. Cell Biol. 136:399–409.
Vietor, I., T. Bader, K. Paiha, and L. A. Huber. 2001. EMBO. Rep 2:306–312.
Huber, D., M. S. Balda, and K. Matter. 2000. J. Biol. Chem. 275:5773–5887.
Huber, D., M. S. Baldo, and K. Matter. 1998. Invasion Metastasis. 18:70–80.
Ruoslahti, E. and M. D. Peirschbacher. 1987. Science 238:491–497.
Blaschuk, O. W., R. Sullivan, S. David, and Y. Pouliot. 1990. Dev.Biol. 139:227–229.
Ando-Akatsuka, Y., M. Saitou, T. Hirase, M. Kishi, A. Sakakibara, M. Itoh, S. Yonemura, M. Furuse, and S. Tsukita. 1996. J. Cell Biol. 133:43–47.
Williams, E., G. Williams, B. J. Gour, O. W. Blaschuk, and P. Doherty. 2000. J. Biol. Chem. 275:4007–4012.
Makrigiannakis, A., G. Coukos, M. Christofidou-Solomidou, B. J. Gour, G. L. Radice, O. Blaschuk, and C. Coutifaris. 1999.Am. J.Pathol. 154:1391–1406.
Wilby, M. J.,E. M. Fok-Seang, B. J. Gour, O. W. Blaschuk, and J.W. Fawcett. 1999. Mol. Cell Neurosci. 14:66–84.
Yoshida, N., D. N. Granger, D. C. Anderson, R. Rothlein, C. Lane, and P. R. Kvietys. 1992. Am. J. Physiol. 262:G1891–1898.
Maruo, N., I. Morita, M. Shirao, and S. Murota. 1992. Endocrinology. 131:710–714.
Kevil, C. G., D. K. Payne, E. Mire, and J. S. Alexander. 1998. J.Biol. Chem. 273:15099–15103.
Bazzoni, G., E. Dejana, and M. G. Lampugnani. 1999. Curr. Opin.Cell Biol. 11:573–581.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Blaschuk, O.W., Oshima, T., Gour, B.J. et al. Identification of an Occludin Cell Adhesion Recognition Sequence. Inflammation 26, 193–198 (2002). https://doi.org/10.1023/A:1016571830091
Issue date:
DOI: https://doi.org/10.1023/A:1016571830091
