Figure 4. Circulating SARS-CoV-2 antigen. Free and total S1 and spike levels (A) measured in the plasma of the vaccinated control (n=44) and myocarditis (n=16) cohorts. Antigen data corresponding to all individuals who received the BNT162b2 vaccine (● ; n=59) and those who received the Moderna vaccine (○; n=2). Free antigen is measured by directly diluting patient plasma, whereas total antigen is measured after treating the plasma with dithiothreitol (DTT) to denature any antigen-bound antibodies. Free and total antigen levels vs days after vaccination (B). Longitudinal measurements associated with the same myocarditis patient are connected with lines. All data points represent mean values for duplicate measurements. Dashed gray lines indicate the limit of detection for each assay. SARS-CoV-2 indicates severe acute respiratory syndrome coronavirus 2.
