Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2009 Jun;18(6):1135-8.
doi: 10.1002/pro.125.

Racemic crystallography--easy crystals and easy structures: what's not to like?

Brian W Matthews  1
Affiliations
Comment

Racemic crystallography--easy crystals and easy structures: what's not to like?

Brian W Matthews. Protein Sci. 2009 Jun.
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
Average and root-mean-square values of the difference electron density for plectasin plotted as a function of the distance from the nearest atom in the model. The difference map (Δρ) has coefficients (Fo−Fc), phases from the refined model of plectasin (PDB code 3E7R), and was calculated on a 0.1 Å grid. For each grid point, a search was made for the nearest atom in the model, including ordered solvent. The density values were binned according to the distance to the nearest atom and the average (red) and rms (green) values plotted in the figure. The histogram also shows the number of grid points in each bin (blue). The plot shown is for the difference density map calculated at 1.5 Å resolution. This is not the outer limit of the data but was used because the X-ray observations are very complete and most of the phases should be correct. Maps at higher resolution give fairly similar histograms as do SigmaA-weighted Fo−Fc maps (not shown).
See this image and copyright information in PMC

Comment on

References

    1. Mandal K, Pentelute B, Tereshko V, Thammavongsa V, Schneewind O, Kossiakoff A, Kent S. Racemic crystallography of synthetic protein enantiomers used to determine the X-ray structure of plectasin by direct methods. Protein Sci. 2009;18:1146–1154. - PMC - PubMed
    1. Zawadzke LE, Berg JM. The structure of a centrosymmetric protein crystal. Proteins. 1993;16:301–305. - PubMed
    1. Pentelute BL, Gates ZP, Tereshko V, Dashnau JL, Vanderkooi JM, Kossiakoff AA, Kent SBH. X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers. J Am Chem Soc. 2008;130:9695–9701. - PMC - PubMed
    1. Wukovitz SW, Yeates TO. Why protein crystals favour some space-groups over others. Nat Struct Biol. 1995;2:1062–1067. - PubMed
    1. Doi M, Ishibe A, Shinozaki H, Urata H, Inoue M, Ishida T. Conserved and novel structural characteristics of enantiomorphic Leu-enkephalin. Int J Peptide Proteins Res. 1994;41:325–331. - PubMed

LinkOut - more resources