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. 2015 Oct:117:74-7.
doi: 10.1016/j.mimet.2015.07.009. Epub 2015 Jul 14.

A method to analyze, sort, and retain viability of obligate anaerobic microorganisms from complex microbial communities

Anne W Thompson  1 , Matthew J Crow  2 , Brian Wadey  2 , Christina Arens  3 , Serdar Turkarslan  3 , Sergey Stolyar  3 , Nicholas Elliott  4 , Timothy W Petersen  2 , Ger van den Engh  5 , David A Stahl  4 , Nitin S Baliga  6
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Free article

A method to analyze, sort, and retain viability of obligate anaerobic microorganisms from complex microbial communities

Anne W Thompson et al. J Microbiol Methods. 2015 Oct.
Free article

Abstract

A high speed flow cytometric cell sorter was modified to maintain a controlled anaerobic environment. This technology enabled coupling of the precise high-throughput analytical and cell separation capabilities of flow cytometry to the assessment of cell viability of evolved lineages of obligate anaerobic organisms from cocultures.

Keywords: Anaerobic microorganism; Controlled-environment sorting; Flow cytometry; Single cells.

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