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. 2017 Jul;88(3):222-224.
doi: 10.1016/j.diagmicrobio.2017.04.008. Epub 2017 Apr 26.

Bactec™ blood culture bottles allied to MALDI-TOF mass spectrometry: rapid etiologic diagnosis of bacterial endophthalmitis

Tatiana Tanaka  1 , Luiza Manhezi de Freitas Oliveira  2 , Bruno Fortaleza de Aquino Ferreira  2 , Juliana Mika Kato  2 , Flavia Rossi  3 , Karoline de Lemes Giuntini Correa  3 , Sergio Luis Gianotti Pimentel  2 , Joyce Hisae Yamamoto  2 , João Nóbrega Almeida Junior  4
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Bactec™ blood culture bottles allied to MALDI-TOF mass spectrometry: rapid etiologic diagnosis of bacterial endophthalmitis

Tatiana Tanaka et al. Diagn Microbiol Infect Dis. 2017 Jul.

Abstract

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been used for direct identification of pathogens from blood-inoculated blood culture bottles (BCBs). We showed that MALDI-TOF MS is an useful technique for rapid identification of the causative agents of endophthalmitis from vitreous humor-inoculated BCBs with a simple protocol.

Keywords: Blood culture bottle; Diagnosis; Endophthalmitis; MALDI-TOF mass spectrometry.

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