Structural basis for adaptation of lactobacilli to gastrointestinal mucus

Etzold, Sabrina; Kober, Olivia; MacKenzie, Donald; Tailford, Louise E.; Gunning, A. Patrick; Walshaw, John; Hemmings, Andrew M.; Juge, Nathalie

doi:10.1111/1462-2920.12377

Environmental Microbiology

2014

DOI: 10.1111/1462-2920.12377

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Structural basis for adaptation of lactobacilli to gastrointestinal mucus

Sabrina Etzold

¹

,

Olivia Kober

²

,

Donald MacKenzie

³

et al.

Abstract: SummaryThe mucus layer covering the gastrointestinal (GI) epithelium is critical in selecting and maintaining homeostatic interactions with our gut bacteria. However, the underpinning mechanisms of these interactions are not understood. Here, we provide structural and functional insights into the canonical mucus-binding protein (MUB), a multi-repeat cellsurface adhesin found in Lactobacillus inhabitants of the GI tract. X-ray crystallography together with small-angle X-ray scattering demonstrated a 'beads on a… Show more

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Cited by 103 publications

(112 citation statements)

References 85 publications

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“…Notably, in our experiment, mucus secreted by HT29-MTX cells did not form a uniform layer over the cell surface but rather a patchy structure with thickness of up to 5 μm. This observation is in agreement with previous studies in which mucus patches were visualised in this in vitro model (Coïc et al 2012; Sperandio et al 2013; Etzold et al 2014). However, it was also reported that mucus secreted by HT29-MTX cells formed a dense gel layer entirely covering the cell surface (Coconnier et al 1992; Gouyer et al 2001; Gibbins et al 2015).…”

Section: Discussionsupporting

confidence: 94%

Contribution of plasmid-encoded peptidase S8 (PrtP) to adhesion and transit in the gut of Lactococcus lactis IBB477 strain

Radziwill-Bienkowska

¹

,

Robert

²

,

Drabot

³

et al. 2017

Appl Microbiol Biotechnol

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…Notably, in our experiment, mucus secreted by HT29-MTX cells did not form a uniform layer over the cell surface but rather a patchy structure with thickness of up to 5 μm. This observation is in agreement with previous studies in which mucus patches were visualised in this in vitro model (Coïc et al 2012; Sperandio et al 2013; Etzold et al 2014). However, it was also reported that mucus secreted by HT29-MTX cells formed a dense gel layer entirely covering the cell surface (Coconnier et al 1992; Gouyer et al 2001; Gibbins et al 2015).…”

Section: Discussionsupporting

confidence: 94%

Contribution of plasmid-encoded peptidase S8 (PrtP) to adhesion and transit in the gut of Lactococcus lactis IBB477 strain

Radziwill-Bienkowska

¹

,

Robert

²

,

Drabot

³

et al. 2017

Appl Microbiol Biotechnol

Get access via publisher Add to collection Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…We showed that mucus did not form a uniform layer above the cell surface, but rather a patchy structure with a thickness of less than 5 µm. Even though mucus secreted by HT29-MTX cells has been described to form a dense gel layer, in some cases entirely covering the cell surface [ 40 – 42 ], our observations are in agreement with previous studies in which mucus patches were visualized instead [ 33 , 43 – 45 ]. Here, we found that food-grade TiO 2 particles formed sparsely distributed clusters throughout the sample undoubtedly embedded in these mucus “islands”.…”

Section: Discussionsupporting

confidence: 92%

Food-grade TiO2 is trapped by intestinal mucus in vitro but does not impair mucin O-glycosylation and short-chain fatty acid synthesis in vivo: implications for gut barrier protection

Talbot

¹

,

Radziwill-Bienkowska

²

,

Kamphuis

³

et al. 2018

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…The presence of multiple binding sites along the length of MUB protein may strengthen binding and confine the bacteria to the outer mucus layer. These data strongly support the binding model we previously proposed between L. reuteri ATCC 53608 MUB and mucin [26]. Such a mechanism is reminiscent of pili-mediated Lactobacillus rhamnosus GG (LGG) interactions with biotic (mucin, intestinal cells) or abiotic (hydrophobic monolayers) surfaces [13,57].…”

Section: Discussionsupporting

confidence: 86%

“…Our data provide evidence in support of the multiple binding model of MUB to terminal sugars present on the glycan antennae of the mucin chains [26]. Binding was decreased (but not abolished) by competition with free sialylated ligands, suggesting that higher concentrations of sugars may be required for inhibition or that MUB may recognize additional sugars on the mucin glycan chains.…”

Section: Discussionsupporting

confidence: 81%

Use of Atomic Force Microscopy to Study the Multi-Modular Interaction of Bacterial Adhesins to Mucins

Gunning

¹

,

Kavanaugh

²

,

Thursby

³

et al. 2016

IJMS

Self Cite

Get access via publisher Add to collection Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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