Unraveling the Substrate Recognition Mechanism and Specificity of the Unusual Glycosyl Hydrolase Family 29 BT2192 from <i>Bacteroides thetaiotaomicron</i>

Guillotin, Laure; Lafite, Pierre; Daniellou, Richard

doi:10.1021/bi400951q

Biochemistry

2014

DOI: 10.1021/bi400951q

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Unraveling the Substrate Recognition Mechanism and Specificity of the Unusual Glycosyl Hydrolase Family 29 BT2192 from Bacteroides thetaiotaomicron

Laure Guillotin

¹

,

Pierre Lafite

²

,

Richard Daniellou

³

Abstract: Glycosyl hydrolase (GH) family 29 (CAZy database) consists of retaining α-l-fucosidases. We have identified BT2192, a protein from Bacteroides thetaiotaomicron, as the first GH29 representative exhibiting both weak α-l-fucosidase and β-d-galactosidase activities. Determination and analysis of X-ray structures of BT2192 in complex with β-d-galactoside competitive inhibitors showed a new binding mode different from that of known GH29 enzymes. Three point mutations, specific to BT2192, prevent the canonical GH29 … Show more

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Cited by 22 publications

(18 citation statements)

References 49 publications

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“…Of note, GH95 enzymes, also referred as 1,2-α-L-fucosidases (EC 3.2.1.63), specifically target α-1,2-fucosidic linkages 44,46 , thus corroborating our observation of FumA1 showing preference towards 2′-fucosyllactose. In contrast, FumA2 clusters with other previously characterized GH29 α-fucosidases, including BT_2970, BT_2192 and BT3798 from Bacteroides thetaiotaomicron VPI-5482 47–49 , Blon_2336 from Bifidobacterium longum subsp. infantis ATCC 15697 44 , TM0306 from Thermotoga maritima 50 and Afc2 from Clostridium perfrigens 46 .…”

Section: Resultsmentioning

confidence: 80%

Metabolism of the predominant human milk oligosaccharide fucosyllactose by an infant gut commensal

James

¹

,

Bottacini

²

,

Serrano-Contreras

³

et al. 2019

Sci Rep

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…Of note, GH95 enzymes, also referred as 1,2-α-L-fucosidases (EC 3.2.1.63), specifically target α-1,2-fucosidic linkages 44,46 , thus corroborating our observation of FumA1 showing preference towards 2′-fucosyllactose. In contrast, FumA2 clusters with other previously characterized GH29 α-fucosidases, including BT_2970, BT_2192 and BT3798 from Bacteroides thetaiotaomicron VPI-5482 47–49 , Blon_2336 from Bifidobacterium longum subsp. infantis ATCC 15697 44 , TM0306 from Thermotoga maritima 50 and Afc2 from Clostridium perfrigens 46 .…”

Section: Resultsmentioning

confidence: 80%

Metabolism of the predominant human milk oligosaccharide fucosyllactose by an infant gut commensal

James

¹

,

Bottacini

²

,

Serrano-Contreras

³

et al. 2019

Sci Rep

Get access via publisher Add to collection Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…infantis and B. thetaiotaomicron, with C␣ r.m.s.d. values of 2.9, 2.9, and 3.5 Å of 299, 305, and 304 residues aligned, respectively (PDB codes 3MO4, 3EYP, and 3GZA) (19,61). 3 Both the overall folds of the N-terminal domains and active site residues are well conserved between FgFCO1 and bacterial homologues (Fig.…”

Section: Resultsmentioning

confidence: 98%

Structure and Substrate Specificity of a Eukaryotic Fucosidase from Fusarium graminearum

Cao

¹

,

Walton

²

,

Brumm

³

2014

Journal of Biological Chemistry

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…The catalytic N-terminal domain exhibits the familiar TIMbarrel fold reported for other GH29 enzymes (Guillotin et al, 2014;Lammerts van Bueren et al, 2010). However, -strands 5 and 6 are offset, with only one intra-main-chain hydrogen bond between them.…”

Section: Structure and Functionmentioning

confidence: 84%

The structure of a glycoside hydrolase 29 family member from a rumen bacterium reveals unique, dual carbohydrate-binding domains

Summers

¹

,

Moon

²

,

Atua

³

et al. 2016

Acta Crystallogr F Struct Biol Commun

Get access via publisher Add to collection Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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