A pipeline for developing and testing staining protocols for flow cytometry, demonstrated with SYBR Green I and propidium iodide viability staining

Neščerecka, Alīna; Hammes, Frederik; Juhna, Tālis

doi:10.1016/j.mimet.2016.10.022

Journal of Microbiological Methods

2016

DOI: 10.1016/j.mimet.2016.10.022

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A pipeline for developing and testing staining protocols for flow cytometry, demonstrated with SYBR Green I and propidium iodide viability staining

Alīna Neščerecka

¹

,

Frederik Hammes

²

,

Tālis Juhna

³

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Cited by 99 publications

(64 citation statements)

References 46 publications

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“…Although time did not have a significant effect on either dye in this study, Nescerecka et al [ 26 ] showed that it was the most significant factor affecting intact bacteria cell concentration in their study on bacteria viability, using SYBR Green I and Propidium Iodide.…”

Section: Resultscontrasting

confidence: 53%

Optimized Protocol for Microalgae DNA Staining with SYTO9/SYBR Green I, Based on Flow Cytometry and RSM Methodology: Experimental Design, Impacts and Validation

Ihadjadene

¹

,

Walther

²

,

Krujatz

³

2022

MPs

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…Although time did not have a significant effect on either dye in this study, Nescerecka et al [ 26 ] showed that it was the most significant factor affecting intact bacteria cell concentration in their study on bacteria viability, using SYBR Green I and Propidium Iodide.…”

Section: Resultscontrasting

confidence: 53%

Optimized Protocol for Microalgae DNA Staining with SYTO9/SYBR Green I, Based on Flow Cytometry and RSM Methodology: Experimental Design, Impacts and Validation

Ihadjadene

¹

,

Walther

²

,

Krujatz

³

2022

MPs

Get access via publisher Add to collection Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…Furthermore, a notable alternative method is the ATP test, a process of rapidly measuring actively growing microorganisms through ATP detection (a molecule found in and around living cells) in the water sample. Studies published by [59,60] confirm the effectiveness of this method. Since ATP is quantified by measuring the light produced through its reaction with the naturally occurring enzyme luciferase using a luminometer, the amount of light produced is directly proportional to the amount of ATP present in the sample.…”

Section: Discussionmentioning

confidence: 58%

Optimization of Quantitative Analysis of Biofilm Cell from Pipe Materials

Papciak

¹

,

Domoń

²

,

Zdeb

³

et al. 2021

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…As found by Hou et al and Nescerecka et al [ 87 , 88 ], the saturated bacterial precipitate resuspended in M9 medium to an OD 600 of 0.2. With or without 40 mM NAC was used to treat bacteria.…”

Section: Methodsmentioning

confidence: 71%

N-acetylcysteine promotes doxycycline resistance in the bacterial pathogen Edwardsiella tarda

Guo

¹

,

Xu

²

,

Zhong

³

et al. 2024

Get access via publisher Add to collection Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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