Secreted Effectors Encoded within and outside of the Francisella Pathogenicity Island Promote Intramacrophage Growth

Eshraghi, Aria; Walls, Alexandra C.; Ledvina, Hannah E.; Miller, Cheryl N.; Ramsey, Kathryn M.; Whitney, John C.; Radey, Matthew C.; Peterson, S. Brook; Ruhland, Brittany R.; Tran, Bao; Goo, Young Ah; Goodlett, David R.; Dove, Simon L.; Celli, Jean; Veesler, David; Mougous, Joseph D.

doi:10.1016/j.chom.2016.10.008

Cell Host & Microbe

2016

DOI: 10.1016/j.chom.2016.10.008

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Secreted Effectors Encoded within and outside of the Francisella Pathogenicity Island Promote Intramacrophage Growth

Aria Eshraghi

¹

,

Alexandra C. Walls

²

,

Hannah E. Ledvina

³

et al.

Abstract: Summary The intracellular bacterial pathogen Francisella tularensis causes tularemia, a zoonosis that can be fatal. The type VI secretion system (T6SS) encoded by the Francisella pathogenicity island (FPI) is critical for the virulence of this organism. Existing studies suggest that the complete repertoire of T6SS effectors delivered to host cells is encoded by the FPI. Using a proteome-wide approach, we discovered that the FPI-encoded T6SS exports at least three effectors encoded outside of the island. These … Show more

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Cited by 74 publications

(121 citation statements)

References 52 publications

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“…Table 1 shows the 25 most abundant proteins detected. The proteins include many that were previously identified as secreted by Francisella, supporting our approach (19,20,(36)(37)(38)(39). The KatG catalase was the sixth most abundant protein detected.…”

Section: Resultssupporting

confidence: 78%

Protein secretion by the type IV pilus machinery in Francisella tularensis

DeRosa

¹

,

Benziger

²

,

Sampath

³

et al. 2026

Preprint

0

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…Table 1 shows the 25 most abundant proteins detected. The proteins include many that were previously identified as secreted by Francisella, supporting our approach (19,20,(36)(37)(38)(39). The KatG catalase was the sixth most abundant protein detected.…”

Section: Resultssupporting

confidence: 78%

Protein secretion by the type IV pilus machinery in Francisella tularensis

DeRosa

¹

,

Benziger

²

,

Sampath

³

et al. 2026

Preprint

0

Get access via publisher Add to collection Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…The additional genomes that have been sequenced upon completion of this study may add to this complexity. The lack of pdpC and pdpD in certain strains was reported previously (Eshraghi et al, 2016). The two genes have previously been suggested to encode effector proteins, in fact, pdpD was identified as an effector also in our computational screen.…”

Section: Discussionsupporting

confidence: 78%

Exploring the Diversity Within the Genus Francisella – An Integrated Pan-Genome and Genome-Mining Approach

Kumar

¹

,

Bröms

²

,

Sjöstedt

³

2020

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…In F. tularensis LVS, there was a slight, but statistically significant, attenuation of virulence in an in vivo chicken embryo infection model. We did not observe any attenuation in the two in vitro cell lines tested; however, it is possible that, as shown previously (37,38), functional redundancy with other T6SS ii effectors, or even other virulence factors, masks the effects of the deletion of opiA under the specific conditions tested.…”

Section: Discussionsupporting

confidence: 48%

“…We also showed that a ΔopiA strain was attenuated in a chicken embryo infection assay, consistent with the role previously identified for opiA in other Francisella spp. in pathogenesis (37,38). However, our data also demonstrated that opiA influences the growth, cell size, viability, and aminoglycoside resistance of F. tularensis LVS, identifying a novel role for opiA, outside the context of infection.…”

Section: Discussionmentioning

confidence: 55%

OpiA, a Type Six Secretion System Substrate, Localizes to the Cell Pole and Plays a Role in Bacterial Growth and Viability inFrancisella tularensisLVS

Cantlay

¹

,

Haggerty

²

,

Horzempa

³

2020

Get access via publisher Add to collection Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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