Two groups report culture conditions for long-term in vitro growth of intestinal tissue from the mouse.
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References
Sato, T. et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459, 262–265 (2009).
Ootani, A. et al. Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche. Nat. Med. 15, 701–706 (2009).
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de Souza, N. A question of culture. Nat Methods 6, 481 (2009). https://doi.org/10.1038/nmeth0709-481
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DOI: https://doi.org/10.1038/nmeth0709-481